Review



notch2 sirna  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Santa Cruz Biotechnology notch2 sirna
    Notch2 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/notch2/Notch+2+siRNA/pm42830147-156-0-2
    Average 93 stars, based on 19 article reviews
    notch2 sirna - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: A NOTCH3-CXCL12-driven myeloma-tumor niche signaling axis promotes chemoresistance in multiple myeloma.
    Article Snippet: Proteins were transferred to PVDF membranes using a semidry blotter (Bio-Rad) and incubated in a blocking solution (5% nonfat dry milk in TBS containing 0.1% Tween-20) for 1 hour to reduce nonspecific binding. .. Immunoblots were performed using anti-GAPDH, pCXCL12, AKT, pAKT, ERK, pERK, NOTCH1, NOTCH2, and NOTCH3 antibodies (1:1000) followed by goat anti-rabbit secondary antibodies, conjugated to horseradish peroxidase (1:2000) in 5% milk (Santa Cruz Biotechnology). .. Western blots were developed using an enhanced chemiluminescence detection assay following the manufacturer's directions (BioRad).

    Article Title: A NOTCH3-CXCL12-driven myeloma-tumor niche signaling axis promotes chemoresistance in multiple myeloma
    Article Snippet: Proteins were transferred to PVDF membranes using a semidry blotter (Bio-Rad) and incubated in a blocking solution (5% nonfat dry milk in TBS containing 0.1% Tween-20) for 1 hour to reduce nonspecific binding. .. Immunoblots were performed using anti-GAPDH, pCXCL12, AKT, pAKT, ERK, pERK, NOTCH1, NOTCH2, and NOTCH3 antibodies (1:1000) followed by goat anti-rabbit secondary antibodies, conjugated to horseradish peroxidase (1:2000) in 5% milk (Santa Cruz Biotechnology). .. Western blots were developed using an enhanced chemiluminescence detection assay following the manufacturer's directions (Bio- Rad).

    Saline:

    Article Title: Deciphering the Contribution of Circular RNAs to Age‐Related Decline in Sertoli Cell Survivor
    Article Snippet: Then, proteins were transferred to a polyvinylidene difluoride membrane (GE Healthcare, Milano, Italy) at 280 mA for 2.5 h at 4°C. .. The filters were blocked [5% nonfat milk, 0.25% Tween‐20 in Tris‐buffered saline (TBS, pH 7.6)] and incubated with different primary antibodies, all diluted 1:1000 [FUS antibody (PA5‐52610; Invitrogen, Milano, Italy), SOX9 (sc‐166505; Santa Cruz Biotechnology, Heidelberg, Germany), SOX8 (sc‐374445; Santa Cruz Biotechnology, Heidelberg, Germany), NOTCH2 (sc‐518049; Santa Cruz Biotechnology, Heidelberg, Germany), WT1 (sc‐7385; Santa Cruz Biotechnology, Heidelberg, Germany), DDX4 (MA5‐15565; Invitrogen, Milano, Italy), MAGE‐A4 (PA5‐72867; Invitrogen, Milano, Italy), SCP3 (PA1‐16766; Invitrogen, Milano, Italy), and TUBULIN (ab15246; Abcam, Cambridge, UK)]. .. Total RNA was extracted from (i) aliquots of cauda SPZ collected from Young and Aged mice ( n = 6 for each experimental group); (ii) reproductive tissues (testis and epididymis) collected from Young ( n = 6) and Aged ( n = 6) mice; (iii) TM4 cells ± siFUS ( n = 6 for each experimental group); (iv) aliquots of A‐ and B‐SPZ collected from normozoospermic men ( n = 6 for each experimental group); and (v) aliquots of A‐SPZ collected from Young and Aged men ( n = 6 for each experimental group) using TRIzol Reagent (Invitrogen Life Technologies, Paisley, UK) following the manufacturer's instructions.

    Article Title: Deciphering the Contribution of Circular RNAs to Age-Related Decline in Sertoli Cell Survivor.
    Article Snippet: Then, proteins were transferred to a polyvinylidene difluoride membrane (GE Healthcare, Milano, Italy) at 280 mA for 2.5 h at 4°C. .. The filters were blocked [5% nonfat milk, 0.25% Tween- 20 in Tris- buffered saline (TBS, pH 7.6)] and incubated with different primary antibodies, all diluted 1:1000 [FUS antibody (PA5- 52610; Invitrogen, Milano, Italy), SOX9 (sc- 166505; Santa Cruz Biotechnology, Heidelberg, Germany), SOX8 (sc- 374445; Santa Cruz Biotechnology, Heidelberg, Germany), NOTCH2 (sc- 518049; Santa Cruz Biotechnology, Heidelberg, Germany), WT1 (sc- 7385; Santa Cruz Biotechnology, Heidelberg, Germany), DDX4 (MA5- 15565; Invitrogen, Milano, Italy), MAGE- A4 (PA5- 72867; Invitrogen, Milano, Italy), SCP3 (PA1- 16766; Invitrogen, Milano, Italy), and TUBULIN (ab15246; Abcam, Cambridge, UK)]. .. Total RNA was extracted from (i) aliquots of cauda SPZ collected from Young and Aged mice (n = 6 for each experimental group); (ii) reproductive tissues (testis and epididymis) collected from Young (n = 6) and Aged (n = 6) mice; (iii) TM4 cells ± siFUS (n = 6 for each experimental group); (iv) aliquots of A- and B- SPZ collected from normozoospermic men (n = 6 for each experimental group); and (v) aliquots of A- SPZ collected from Young and Aged men (n = 6 for each experimental group) using TRIzol Reagent (Invitrogen Life Technologies, Paisley, UK) following the manufacturer's instructions.

    Incubation:

    Article Title: Deciphering the Contribution of Circular RNAs to Age‐Related Decline in Sertoli Cell Survivor
    Article Snippet: Then, proteins were transferred to a polyvinylidene difluoride membrane (GE Healthcare, Milano, Italy) at 280 mA for 2.5 h at 4°C. .. The filters were blocked [5% nonfat milk, 0.25% Tween‐20 in Tris‐buffered saline (TBS, pH 7.6)] and incubated with different primary antibodies, all diluted 1:1000 [FUS antibody (PA5‐52610; Invitrogen, Milano, Italy), SOX9 (sc‐166505; Santa Cruz Biotechnology, Heidelberg, Germany), SOX8 (sc‐374445; Santa Cruz Biotechnology, Heidelberg, Germany), NOTCH2 (sc‐518049; Santa Cruz Biotechnology, Heidelberg, Germany), WT1 (sc‐7385; Santa Cruz Biotechnology, Heidelberg, Germany), DDX4 (MA5‐15565; Invitrogen, Milano, Italy), MAGE‐A4 (PA5‐72867; Invitrogen, Milano, Italy), SCP3 (PA1‐16766; Invitrogen, Milano, Italy), and TUBULIN (ab15246; Abcam, Cambridge, UK)]. .. Total RNA was extracted from (i) aliquots of cauda SPZ collected from Young and Aged mice ( n = 6 for each experimental group); (ii) reproductive tissues (testis and epididymis) collected from Young ( n = 6) and Aged ( n = 6) mice; (iii) TM4 cells ± siFUS ( n = 6 for each experimental group); (iv) aliquots of A‐ and B‐SPZ collected from normozoospermic men ( n = 6 for each experimental group); and (v) aliquots of A‐SPZ collected from Young and Aged men ( n = 6 for each experimental group) using TRIzol Reagent (Invitrogen Life Technologies, Paisley, UK) following the manufacturer's instructions.

    Article Title: Deciphering the Contribution of Circular RNAs to Age-Related Decline in Sertoli Cell Survivor.
    Article Snippet: Then, proteins were transferred to a polyvinylidene difluoride membrane (GE Healthcare, Milano, Italy) at 280 mA for 2.5 h at 4°C. .. The filters were blocked [5% nonfat milk, 0.25% Tween- 20 in Tris- buffered saline (TBS, pH 7.6)] and incubated with different primary antibodies, all diluted 1:1000 [FUS antibody (PA5- 52610; Invitrogen, Milano, Italy), SOX9 (sc- 166505; Santa Cruz Biotechnology, Heidelberg, Germany), SOX8 (sc- 374445; Santa Cruz Biotechnology, Heidelberg, Germany), NOTCH2 (sc- 518049; Santa Cruz Biotechnology, Heidelberg, Germany), WT1 (sc- 7385; Santa Cruz Biotechnology, Heidelberg, Germany), DDX4 (MA5- 15565; Invitrogen, Milano, Italy), MAGE- A4 (PA5- 72867; Invitrogen, Milano, Italy), SCP3 (PA1- 16766; Invitrogen, Milano, Italy), and TUBULIN (ab15246; Abcam, Cambridge, UK)]. .. Total RNA was extracted from (i) aliquots of cauda SPZ collected from Young and Aged mice (n = 6 for each experimental group); (ii) reproductive tissues (testis and epididymis) collected from Young (n = 6) and Aged (n = 6) mice; (iii) TM4 cells ± siFUS (n = 6 for each experimental group); (iv) aliquots of A- and B- SPZ collected from normozoospermic men (n = 6 for each experimental group); and (v) aliquots of A- SPZ collected from Young and Aged men (n = 6 for each experimental group) using TRIzol Reagent (Invitrogen Life Technologies, Paisley, UK) following the manufacturer's instructions.



    Similar Products

    notch2 antibody stainings  (Miltenyi Biotec)
    90
    Miltenyi Biotec notch2 antibody stainings
    Notch2 Antibody Stainings, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/notch2/Notch2+Antibody%2C+anti-human/pm42030954-336-4-18
    Average 90 stars, based on 1 article reviews
    notch2 antibody stainings - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    notch2 sirna  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology notch2 sirna
    Notch2 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/notch2/Notch+2+siRNA/pm42830147-156-0-2
    Average 93 stars, based on 1 article reviews
    notch2 sirna - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    anti notch2  (ABclonal Biotechnology)
    94
    ABclonal Biotechnology anti notch2
    Anti Notch2, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/notch2/NOTCH2+Rabbit+pAb/pmc13531868-188-46-48
    Average 94 stars, based on 1 article reviews
    anti notch2 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    notch2  (Santa Cruz Biotechnology)
    91
    Santa Cruz Biotechnology notch2
    Transcriptomic profiling reveals significant enrichment of lncRNA OTUD6B-AS1 in exosomes derived from LPS-stimulated EECs. (A, B) LPS-exo was treated with RNase A alone or in combination with Triton X-100 for 4 h, and then co-incubated with macrophages. Relative expression levels of iNOS (A) and Arg1 (B) in macrophages. (C) Schematic overview of the RNA sequencing and analysis workflow. (D) Volcano plot showing differentially expressed lncRNAs in LPS-exo compared to Control-exo. (E, F) Gene Ontology (GO) biological process enrichment analysis (E) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis (F) of the differentially expressed lncRNAs. (G) qPCR validation of the 6 upregulated lncRNAs in Control-exo and LPS-exo. (H) LPS-exo was treated with RNase A alone or in combination with Triton X-100 for 4 h, and then co-incubated with macrophages. Relative mRNA expression level of lncRNA OTUD6B-AS1 in macrophages. (I) A proposed competing endogenous RNA (ceRNA) network involving lncRNA OTUD6B-AS1, miR-128, and <t>Notch2.</t> (J) Relative mRNA expression level of lncRNA OTUD6B-AS1 in control and LPS-stimulated EECs. (K, L) RNA fluorescence in situ hybridization (RNA-FISH) showing the subcellular localization of lncRNA OTUD6B-AS1 (red) in EECs (K) and its quantitative cytoplasmic/nuclear distribution (L). Nuclei were stained with DAPI (blue). (M – P) Relative mRNA expression levels of lncRNA OTUD6B-AS1 (M − O) and miR-128 (P) in endometrial tissues from healthy cows and cows with endometritis, as determined by qPCR (O, P) and RNA-FISH (M) with quantification (N). (Q) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 protein levels in endometrial tissues. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Notch2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/notch2/Diphtheria+Toxin+A+Antibody/pmc13022686-110-42-43
    Average 91 stars, based on 1 article reviews
    notch2 - by Bioz Stars, 2026-10
    91/100 stars
    		  Buy from Supplier
    notch2  (ABclonal Biotechnology)
    94
    ABclonal Biotechnology notch2
    Transcriptomic profiling reveals significant enrichment of lncRNA OTUD6B-AS1 in exosomes derived from LPS-stimulated EECs. (A, B) LPS-exo was treated with RNase A alone or in combination with Triton X-100 for 4 h, and then co-incubated with macrophages. Relative expression levels of iNOS (A) and Arg1 (B) in macrophages. (C) Schematic overview of the RNA sequencing and analysis workflow. (D) Volcano plot showing differentially expressed lncRNAs in LPS-exo compared to Control-exo. (E, F) Gene Ontology (GO) biological process enrichment analysis (E) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis (F) of the differentially expressed lncRNAs. (G) qPCR validation of the 6 upregulated lncRNAs in Control-exo and LPS-exo. (H) LPS-exo was treated with RNase A alone or in combination with Triton X-100 for 4 h, and then co-incubated with macrophages. Relative mRNA expression level of lncRNA OTUD6B-AS1 in macrophages. (I) A proposed competing endogenous RNA (ceRNA) network involving lncRNA OTUD6B-AS1, miR-128, and <t>Notch2.</t> (J) Relative mRNA expression level of lncRNA OTUD6B-AS1 in control and LPS-stimulated EECs. (K, L) RNA fluorescence in situ hybridization (RNA-FISH) showing the subcellular localization of lncRNA OTUD6B-AS1 (red) in EECs (K) and its quantitative cytoplasmic/nuclear distribution (L). Nuclei were stained with DAPI (blue). (M – P) Relative mRNA expression levels of lncRNA OTUD6B-AS1 (M − O) and miR-128 (P) in endometrial tissues from healthy cows and cows with endometritis, as determined by qPCR (O, P) and RNA-FISH (M) with quantification (N). (Q) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 protein levels in endometrial tissues. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Notch2, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/notch2/NOTCH2+Rabbit+pAb/10__1016_slash_j__jafr__2026__102907-101-16-18
    Average 94 stars, based on 1 article reviews
    notch2 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    notch2 flox flox conditional knockout cko mice  (Mutant Mouse Resource & Research Center)
    86
    Mutant Mouse Resource & Research Center notch2 flox flox conditional knockout cko mice
    Transcriptomic profiling reveals significant enrichment of lncRNA OTUD6B-AS1 in exosomes derived from LPS-stimulated EECs. (A, B) LPS-exo was treated with RNase A alone or in combination with Triton X-100 for 4 h, and then co-incubated with macrophages. Relative expression levels of iNOS (A) and Arg1 (B) in macrophages. (C) Schematic overview of the RNA sequencing and analysis workflow. (D) Volcano plot showing differentially expressed lncRNAs in LPS-exo compared to Control-exo. (E, F) Gene Ontology (GO) biological process enrichment analysis (E) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis (F) of the differentially expressed lncRNAs. (G) qPCR validation of the 6 upregulated lncRNAs in Control-exo and LPS-exo. (H) LPS-exo was treated with RNase A alone or in combination with Triton X-100 for 4 h, and then co-incubated with macrophages. Relative mRNA expression level of lncRNA OTUD6B-AS1 in macrophages. (I) A proposed competing endogenous RNA (ceRNA) network involving lncRNA OTUD6B-AS1, miR-128, and <t>Notch2.</t> (J) Relative mRNA expression level of lncRNA OTUD6B-AS1 in control and LPS-stimulated EECs. (K, L) RNA fluorescence in situ hybridization (RNA-FISH) showing the subcellular localization of lncRNA OTUD6B-AS1 (red) in EECs (K) and its quantitative cytoplasmic/nuclear distribution (L). Nuclei were stained with DAPI (blue). (M – P) Relative mRNA expression levels of lncRNA OTUD6B-AS1 (M − O) and miR-128 (P) in endometrial tissues from healthy cows and cows with endometritis, as determined by qPCR (O, P) and RNA-FISH (M) with quantification (N). (Q) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 protein levels in endometrial tissues. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Notch2 Flox Flox Conditional Knockout Cko Mice, supplied by Mutant Mouse Resource & Research Center, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/notch2/cko+conditional+flox+flox+knockout+mice+notch2/pm41912527-300-33-9
    Average 86 stars, based on 1 article reviews
    notch2 flox flox conditional knockout cko mice - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    horseradish peroxidase  (Cell Signaling Technology Inc)
    95
    Cell Signaling Technology Inc horseradish peroxidase
    Transcriptomic profiling reveals significant enrichment of lncRNA OTUD6B-AS1 in exosomes derived from LPS-stimulated EECs. (A, B) LPS-exo was treated with RNase A alone or in combination with Triton X-100 for 4 h, and then co-incubated with macrophages. Relative expression levels of iNOS (A) and Arg1 (B) in macrophages. (C) Schematic overview of the RNA sequencing and analysis workflow. (D) Volcano plot showing differentially expressed lncRNAs in LPS-exo compared to Control-exo. (E, F) Gene Ontology (GO) biological process enrichment analysis (E) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis (F) of the differentially expressed lncRNAs. (G) qPCR validation of the 6 upregulated lncRNAs in Control-exo and LPS-exo. (H) LPS-exo was treated with RNase A alone or in combination with Triton X-100 for 4 h, and then co-incubated with macrophages. Relative mRNA expression level of lncRNA OTUD6B-AS1 in macrophages. (I) A proposed competing endogenous RNA (ceRNA) network involving lncRNA OTUD6B-AS1, miR-128, and <t>Notch2.</t> (J) Relative mRNA expression level of lncRNA OTUD6B-AS1 in control and LPS-stimulated EECs. (K, L) RNA fluorescence in situ hybridization (RNA-FISH) showing the subcellular localization of lncRNA OTUD6B-AS1 (red) in EECs (K) and its quantitative cytoplasmic/nuclear distribution (L). Nuclei were stained with DAPI (blue). (M – P) Relative mRNA expression levels of lncRNA OTUD6B-AS1 (M − O) and miR-128 (P) in endometrial tissues from healthy cows and cows with endometritis, as determined by qPCR (O, P) and RNA-FISH (M) with quantification (N). (Q) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 protein levels in endometrial tissues. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Horseradish Peroxidase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/notch2/Notch2+XP+Rabbit+mAb/pm41897433-100-14-29
    Average 95 stars, based on 1 article reviews
    horseradish peroxidase - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    horseradish peroxidase hrp  (Cell Signaling Technology Inc)
    95
    Cell Signaling Technology Inc horseradish peroxidase hrp
    Transcriptomic profiling reveals significant enrichment of lncRNA OTUD6B-AS1 in exosomes derived from LPS-stimulated EECs. (A, B) LPS-exo was treated with RNase A alone or in combination with Triton X-100 for 4 h, and then co-incubated with macrophages. Relative expression levels of iNOS (A) and Arg1 (B) in macrophages. (C) Schematic overview of the RNA sequencing and analysis workflow. (D) Volcano plot showing differentially expressed lncRNAs in LPS-exo compared to Control-exo. (E, F) Gene Ontology (GO) biological process enrichment analysis (E) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis (F) of the differentially expressed lncRNAs. (G) qPCR validation of the 6 upregulated lncRNAs in Control-exo and LPS-exo. (H) LPS-exo was treated with RNase A alone or in combination with Triton X-100 for 4 h, and then co-incubated with macrophages. Relative mRNA expression level of lncRNA OTUD6B-AS1 in macrophages. (I) A proposed competing endogenous RNA (ceRNA) network involving lncRNA OTUD6B-AS1, miR-128, and <t>Notch2.</t> (J) Relative mRNA expression level of lncRNA OTUD6B-AS1 in control and LPS-stimulated EECs. (K, L) RNA fluorescence in situ hybridization (RNA-FISH) showing the subcellular localization of lncRNA OTUD6B-AS1 (red) in EECs (K) and its quantitative cytoplasmic/nuclear distribution (L). Nuclei were stained with DAPI (blue). (M – P) Relative mRNA expression levels of lncRNA OTUD6B-AS1 (M − O) and miR-128 (P) in endometrial tissues from healthy cows and cows with endometritis, as determined by qPCR (O, P) and RNA-FISH (M) with quantification (N). (Q) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 protein levels in endometrial tissues. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Horseradish Peroxidase Hrp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/notch2/Notch2+XP+Rabbit+mAb/pm41898775-325-0-6
    Average 95 stars, based on 1 article reviews
    horseradish peroxidase hrp - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Transcriptomic profiling reveals significant enrichment of lncRNA OTUD6B-AS1 in exosomes derived from LPS-stimulated EECs. (A, B) LPS-exo was treated with RNase A alone or in combination with Triton X-100 for 4 h, and then co-incubated with macrophages. Relative expression levels of iNOS (A) and Arg1 (B) in macrophages. (C) Schematic overview of the RNA sequencing and analysis workflow. (D) Volcano plot showing differentially expressed lncRNAs in LPS-exo compared to Control-exo. (E, F) Gene Ontology (GO) biological process enrichment analysis (E) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis (F) of the differentially expressed lncRNAs. (G) qPCR validation of the 6 upregulated lncRNAs in Control-exo and LPS-exo. (H) LPS-exo was treated with RNase A alone or in combination with Triton X-100 for 4 h, and then co-incubated with macrophages. Relative mRNA expression level of lncRNA OTUD6B-AS1 in macrophages. (I) A proposed competing endogenous RNA (ceRNA) network involving lncRNA OTUD6B-AS1, miR-128, and Notch2. (J) Relative mRNA expression level of lncRNA OTUD6B-AS1 in control and LPS-stimulated EECs. (K, L) RNA fluorescence in situ hybridization (RNA-FISH) showing the subcellular localization of lncRNA OTUD6B-AS1 (red) in EECs (K) and its quantitative cytoplasmic/nuclear distribution (L). Nuclei were stained with DAPI (blue). (M – P) Relative mRNA expression levels of lncRNA OTUD6B-AS1 (M − O) and miR-128 (P) in endometrial tissues from healthy cows and cows with endometritis, as determined by qPCR (O, P) and RNA-FISH (M) with quantification (N). (Q) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 protein levels in endometrial tissues. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Exosomal lncRNA OTUD6B-AS1 as a pathogenic nanocarrier promotes inflammatory macrophage polarization in endometritis via a targetable ceRNA circuit

    doi: 10.1016/j.mtbio.2026.103027

    Figure Lengend Snippet: Transcriptomic profiling reveals significant enrichment of lncRNA OTUD6B-AS1 in exosomes derived from LPS-stimulated EECs. (A, B) LPS-exo was treated with RNase A alone or in combination with Triton X-100 for 4 h, and then co-incubated with macrophages. Relative expression levels of iNOS (A) and Arg1 (B) in macrophages. (C) Schematic overview of the RNA sequencing and analysis workflow. (D) Volcano plot showing differentially expressed lncRNAs in LPS-exo compared to Control-exo. (E, F) Gene Ontology (GO) biological process enrichment analysis (E) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis (F) of the differentially expressed lncRNAs. (G) qPCR validation of the 6 upregulated lncRNAs in Control-exo and LPS-exo. (H) LPS-exo was treated with RNase A alone or in combination with Triton X-100 for 4 h, and then co-incubated with macrophages. Relative mRNA expression level of lncRNA OTUD6B-AS1 in macrophages. (I) A proposed competing endogenous RNA (ceRNA) network involving lncRNA OTUD6B-AS1, miR-128, and Notch2. (J) Relative mRNA expression level of lncRNA OTUD6B-AS1 in control and LPS-stimulated EECs. (K, L) RNA fluorescence in situ hybridization (RNA-FISH) showing the subcellular localization of lncRNA OTUD6B-AS1 (red) in EECs (K) and its quantitative cytoplasmic/nuclear distribution (L). Nuclei were stained with DAPI (blue). (M – P) Relative mRNA expression levels of lncRNA OTUD6B-AS1 (M − O) and miR-128 (P) in endometrial tissues from healthy cows and cows with endometritis, as determined by qPCR (O, P) and RNA-FISH (M) with quantification (N). (Q) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 protein levels in endometrial tissues. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: The antibodies used include TSG101 (Genuin Biotech, Cat # 51942), CD81 (Santa Cruz Biotechnology, Cat # sc-18877), AKT (abcam, Cat # AB81283), Phospho-AKT (abcam, Cat # AB179463 ), NF-κB p65 (abcam, Cat # AB32536), Phospho-NF-κB p65 (Cell Signaling Technology, Cat # 3033S), Notch2 (Santa Cruz Biotechnology, Cat # sc-51869), RBP-Jκ (Santa Cruz Biotechnology, Cat # sc-271128), and β-actin (Bioss, Cat # bs-0061R).

    Techniques: Derivative Assay, Incubation, Expressing, RNA Sequencing, Control, Biomarker Discovery, Fluorescence, In Situ Hybridization, Staining, Western Blot

    EECs-derived exosomes induce pro-inflammatory macrophage activation via delivery of lncRNA OTUD6B-AS1. (A) Relative mRNA expression level of lncRNA OTUD6B-AS1 in macrophages treated with Control-exo or LPS-exo. (B, C) RNA-FISH images (B) and quantitative analysis (C) showing lncRNA OTUD6B-AS1 (red) transfer to macrophages after co-culture with Control-exo or LPS-exo. Nuclei were stained with DAPI (blue). (D) Relative mRNA expression level of lncRNA OTUD6B-AS1 in macrophages after transfection with lncRNA OTUD6B-AS1 overexpression plasmids (OE-lncRNA) or control plasmids (OE-NC). ( E – I) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 (E), along with immunofluorescence (IF) quantitative analysis of iNOS (F, G) and Arg1 (H, I) protein levels in macrophages after transfection with OE-lncRNA or OE-NC. (J – N) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 (J), along with IF quantitative analysis of iNOS (K, L) and Arg1 (M, N) protein levels in macrophages after lncRNA OTUD6B-AS1 knockdown (si-lncRNA) or control treatment (si-NC). (O) Relative mRNA expression level of lncRNA OTUD6B-AS1 in exosomes isolated from lncRNA OTUD6B-AS1-knockdown LPS-stimulated EECs (si-lncRNA-LPS-exo) or exosomes from siRNA NC-transfected LPS-stimulated EECs (si-NC-LPS-exo). (P – S) IF quantitative analysis of iNOS (P, Q) and Arg1 (R, S) protein levels in macrophages treated with si-lncRNA-LPS-exo or si-NC-LPS-exo. (T) Relative mRNA expression levels of iNOS and Arg1 in macrophages treated with exosomes isolated from control EECs overexpressing lncRNA OTUD6B-AS1 (OE-lncRNA-Control-exo) or exosomes from control plasmids-transfected EECs (OE-NC-Control-exo). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Exosomal lncRNA OTUD6B-AS1 as a pathogenic nanocarrier promotes inflammatory macrophage polarization in endometritis via a targetable ceRNA circuit

    doi: 10.1016/j.mtbio.2026.103027

    Figure Lengend Snippet: EECs-derived exosomes induce pro-inflammatory macrophage activation via delivery of lncRNA OTUD6B-AS1. (A) Relative mRNA expression level of lncRNA OTUD6B-AS1 in macrophages treated with Control-exo or LPS-exo. (B, C) RNA-FISH images (B) and quantitative analysis (C) showing lncRNA OTUD6B-AS1 (red) transfer to macrophages after co-culture with Control-exo or LPS-exo. Nuclei were stained with DAPI (blue). (D) Relative mRNA expression level of lncRNA OTUD6B-AS1 in macrophages after transfection with lncRNA OTUD6B-AS1 overexpression plasmids (OE-lncRNA) or control plasmids (OE-NC). ( E – I) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 (E), along with immunofluorescence (IF) quantitative analysis of iNOS (F, G) and Arg1 (H, I) protein levels in macrophages after transfection with OE-lncRNA or OE-NC. (J – N) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 (J), along with IF quantitative analysis of iNOS (K, L) and Arg1 (M, N) protein levels in macrophages after lncRNA OTUD6B-AS1 knockdown (si-lncRNA) or control treatment (si-NC). (O) Relative mRNA expression level of lncRNA OTUD6B-AS1 in exosomes isolated from lncRNA OTUD6B-AS1-knockdown LPS-stimulated EECs (si-lncRNA-LPS-exo) or exosomes from siRNA NC-transfected LPS-stimulated EECs (si-NC-LPS-exo). (P – S) IF quantitative analysis of iNOS (P, Q) and Arg1 (R, S) protein levels in macrophages treated with si-lncRNA-LPS-exo or si-NC-LPS-exo. (T) Relative mRNA expression levels of iNOS and Arg1 in macrophages treated with exosomes isolated from control EECs overexpressing lncRNA OTUD6B-AS1 (OE-lncRNA-Control-exo) or exosomes from control plasmids-transfected EECs (OE-NC-Control-exo). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: The antibodies used include TSG101 (Genuin Biotech, Cat # 51942), CD81 (Santa Cruz Biotechnology, Cat # sc-18877), AKT (abcam, Cat # AB81283), Phospho-AKT (abcam, Cat # AB179463 ), NF-κB p65 (abcam, Cat # AB32536), Phospho-NF-κB p65 (Cell Signaling Technology, Cat # 3033S), Notch2 (Santa Cruz Biotechnology, Cat # sc-51869), RBP-Jκ (Santa Cruz Biotechnology, Cat # sc-271128), and β-actin (Bioss, Cat # bs-0061R).

    Techniques: Derivative Assay, Activation Assay, Expressing, Control, Co-Culture Assay, Staining, Transfection, Over Expression, Western Blot, Immunofluorescence, Knockdown, Isolation

    lncRNA OTUD6B-AS1 acts as a ceRNA by sponging miR-128 to facilitate pro-inflammatory macrophage activation. (A) Relative mRNA expression level of miR-128 in macrophages treated with Control-exo or LPS-exo. (B) Luciferase reporter assay in HEK293T cells co-transfected with wild-type (WT) or mutant (MUT) lncRNA OTUD6B-AS1 reporter plasmids and miR-128 mimic or mimic NC. (C) RNA pull-down detection of the enrichment of miR-128 to lncRNA OTUD6B-AS1. (D) Ago2 RIP assay analysis of the enrichment of lncRNA OTUD6B-AS1 pulled-down from the Ago2 protein. (E) Relative mRNA expression level of miR-128 in macrophages transfected with OE-NC or OE-lncRNA. (F – J) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 (F), along with immunofluorescence (IF) quantitative analysis of iNOS (G, H) and Arg1 (I, J) protein levels in macrophages co-transfected with OE-lncRNA and miR-128 mimic or mimic NC. (K, L) Relative mRNA expression levels of IL-1β (K) and IL-6 (L) in macrophages co-transfected with OE-lncRNA and miR-128 mimic or mimic NC. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Journal: Materials Today Bio

    Article Title: Exosomal lncRNA OTUD6B-AS1 as a pathogenic nanocarrier promotes inflammatory macrophage polarization in endometritis via a targetable ceRNA circuit

    doi: 10.1016/j.mtbio.2026.103027

    Figure Lengend Snippet: lncRNA OTUD6B-AS1 acts as a ceRNA by sponging miR-128 to facilitate pro-inflammatory macrophage activation. (A) Relative mRNA expression level of miR-128 in macrophages treated with Control-exo or LPS-exo. (B) Luciferase reporter assay in HEK293T cells co-transfected with wild-type (WT) or mutant (MUT) lncRNA OTUD6B-AS1 reporter plasmids and miR-128 mimic or mimic NC. (C) RNA pull-down detection of the enrichment of miR-128 to lncRNA OTUD6B-AS1. (D) Ago2 RIP assay analysis of the enrichment of lncRNA OTUD6B-AS1 pulled-down from the Ago2 protein. (E) Relative mRNA expression level of miR-128 in macrophages transfected with OE-NC or OE-lncRNA. (F – J) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 (F), along with immunofluorescence (IF) quantitative analysis of iNOS (G, H) and Arg1 (I, J) protein levels in macrophages co-transfected with OE-lncRNA and miR-128 mimic or mimic NC. (K, L) Relative mRNA expression levels of IL-1β (K) and IL-6 (L) in macrophages co-transfected with OE-lncRNA and miR-128 mimic or mimic NC. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Article Snippet: The antibodies used include TSG101 (Genuin Biotech, Cat # 51942), CD81 (Santa Cruz Biotechnology, Cat # sc-18877), AKT (abcam, Cat # AB81283), Phospho-AKT (abcam, Cat # AB179463 ), NF-κB p65 (abcam, Cat # AB32536), Phospho-NF-κB p65 (Cell Signaling Technology, Cat # 3033S), Notch2 (Santa Cruz Biotechnology, Cat # sc-51869), RBP-Jκ (Santa Cruz Biotechnology, Cat # sc-271128), and β-actin (Bioss, Cat # bs-0061R).

    Techniques: Activation Assay, Expressing, Control, Luciferase, Reporter Assay, Transfection, Mutagenesis, Western Blot, Immunofluorescence

    Notch2 mediates the regulatory effect of the lncRNA OTUD6B-AS1/miR-128 axis on macrophage activation. (A) Predictive analysis of miR-128 targets using multiple databases. (B) Western blotting analysis of Notch2 protein levels in macrophages treated with Control-exo or LPS-exo. (C) Western blotting analysis of Notch2 protein levels in macrophages transfected with OE-NC or OE-lncRNA. (D – H) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 (D), along with immunofluorescence (IF) quantitative analysis of iNOS (E, F) and Arg1 (G, H) protein levels in macrophages treated with OE-NC or OE-lncRNA and the Notch2 inhibitor DAPT. (I) Luciferase reporter assay in HEK293T cells co-transfected with WT or MUT Notch2 3′UTR reporter plasmids and miR-128 mimic or mimic NC. (J, K) Relative protein (J) and mRNA (K) expression levels of Notch2 in macrophages transfected with miR-128 mimic or mimic NC. (L – P) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 (L), along with IF quantitative analysis of iNOS (M, N) and Arg1 (O, P) protein levels in macrophages co-treated with miR-128 inhibitor or inhibitor NC and DAPT. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Journal: Materials Today Bio

    Article Title: Exosomal lncRNA OTUD6B-AS1 as a pathogenic nanocarrier promotes inflammatory macrophage polarization in endometritis via a targetable ceRNA circuit

    doi: 10.1016/j.mtbio.2026.103027

    Figure Lengend Snippet: Notch2 mediates the regulatory effect of the lncRNA OTUD6B-AS1/miR-128 axis on macrophage activation. (A) Predictive analysis of miR-128 targets using multiple databases. (B) Western blotting analysis of Notch2 protein levels in macrophages treated with Control-exo or LPS-exo. (C) Western blotting analysis of Notch2 protein levels in macrophages transfected with OE-NC or OE-lncRNA. (D – H) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 (D), along with immunofluorescence (IF) quantitative analysis of iNOS (E, F) and Arg1 (G, H) protein levels in macrophages treated with OE-NC or OE-lncRNA and the Notch2 inhibitor DAPT. (I) Luciferase reporter assay in HEK293T cells co-transfected with WT or MUT Notch2 3′UTR reporter plasmids and miR-128 mimic or mimic NC. (J, K) Relative protein (J) and mRNA (K) expression levels of Notch2 in macrophages transfected with miR-128 mimic or mimic NC. (L – P) Western blotting analysis of Notch2, RBP-Jκ, and p-p65 (L), along with IF quantitative analysis of iNOS (M, N) and Arg1 (O, P) protein levels in macrophages co-treated with miR-128 inhibitor or inhibitor NC and DAPT. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Article Snippet: The antibodies used include TSG101 (Genuin Biotech, Cat # 51942), CD81 (Santa Cruz Biotechnology, Cat # sc-18877), AKT (abcam, Cat # AB81283), Phospho-AKT (abcam, Cat # AB179463 ), NF-κB p65 (abcam, Cat # AB32536), Phospho-NF-κB p65 (Cell Signaling Technology, Cat # 3033S), Notch2 (Santa Cruz Biotechnology, Cat # sc-51869), RBP-Jκ (Santa Cruz Biotechnology, Cat # sc-271128), and β-actin (Bioss, Cat # bs-0061R).

    Techniques: Activation Assay, Western Blot, Control, Transfection, Immunofluorescence, Luciferase, Reporter Assay, Expressing

    A proposed model illustrating the exosome-mediated lncRNA OTUD6B-AS1/miR-128/Notch2 axis in aggravating endometritis. Upon LPS-induced damage, endometrial epithelial cells (EECs) release increased exosomes carrying elevated levels of lncRNA OTUD6B-AS1. These exosomes are taken up by endometrial macrophages. The transferred lncRNA OTUD6B-AS1 acts as a molecular sponge to sequester miR-128, leading to the derepression and upregulation of its target gene, Notch2. The enhanced Notch2 signaling subsequently promotes macrophage polarization towards a pro-inflammatory M1 phenotype, characterized by increased NF-κB activation and iNOS expression, thereby exacerbating endometrial inflammation and tissue damage.

    Journal: Materials Today Bio

    Article Title: Exosomal lncRNA OTUD6B-AS1 as a pathogenic nanocarrier promotes inflammatory macrophage polarization in endometritis via a targetable ceRNA circuit

    doi: 10.1016/j.mtbio.2026.103027

    Figure Lengend Snippet: A proposed model illustrating the exosome-mediated lncRNA OTUD6B-AS1/miR-128/Notch2 axis in aggravating endometritis. Upon LPS-induced damage, endometrial epithelial cells (EECs) release increased exosomes carrying elevated levels of lncRNA OTUD6B-AS1. These exosomes are taken up by endometrial macrophages. The transferred lncRNA OTUD6B-AS1 acts as a molecular sponge to sequester miR-128, leading to the derepression and upregulation of its target gene, Notch2. The enhanced Notch2 signaling subsequently promotes macrophage polarization towards a pro-inflammatory M1 phenotype, characterized by increased NF-κB activation and iNOS expression, thereby exacerbating endometrial inflammation and tissue damage.

    Article Snippet: The antibodies used include TSG101 (Genuin Biotech, Cat # 51942), CD81 (Santa Cruz Biotechnology, Cat # sc-18877), AKT (abcam, Cat # AB81283), Phospho-AKT (abcam, Cat # AB179463 ), NF-κB p65 (abcam, Cat # AB32536), Phospho-NF-κB p65 (Cell Signaling Technology, Cat # 3033S), Notch2 (Santa Cruz Biotechnology, Cat # sc-51869), RBP-Jκ (Santa Cruz Biotechnology, Cat # sc-271128), and β-actin (Bioss, Cat # bs-0061R).

    Techniques: Activation Assay, Expressing